Human/Mouse HMGA1B Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5956T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5956T-100UG

Save 15% on Select RUO Reagents. See Details.

All HMGA1B Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse HMGA1B Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse HMGA1B in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HMGA1B
Met1-Glu96
Accession # P17096

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HMGA1B

HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10‑11 kDa member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences. It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well. Human HMGA1B is 96 amino acids (aa) in length and contains three A-T hook DNA-binding domains (aa 21‑31; 42‑52; 67‑78). It is constitutively phosphorylated, and may undergo acetylation. There are three potential splice variants. All three show the same 11 aa insertion after Pro34. In addition, one form shows an additional 56 aa substitution for aa 55‑96, while a second shows a 14 aa substitution for aa 80‑96. Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B.

Long Name

High Mobility Group AT-hook 1B

Entrez Gene IDs

3159 (Human); 15361 (Mouse); 117062 (Rat)

Alternate Names

HMGA1; HMGA1A; HMGA1B; HMGIY; HMG-R; HMG-Y