Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain‑containing protein 2) and JMJ] is a 140‑155 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human Jumonji is 1246 amino acids (aa) in length. It contains an NLS (aa 1‑131), a transcriptional repressor domain (aa 132‑222), a DNA binding region (aa 558‑709) and a nonfunctional histone demethylase domain (aa 916‑1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304‑1151, and a third contains a 22 aa substitution for aa 1‑60, coupled to a 50 aa substitution for aa 949‑1246. Over aa 1‑159, human Jumonji shares 96% aa identity with mouse Jumonji.
Jumonji/JARID2 Antibody
R&D Systems | Catalog # AF6090
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Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Met1-Thr159
Accession # Q92833
Specificity
Clonality
Host
Isotype
Scientific Data Images for Jumonji/JARID2 Antibody
Detection of Human Jumonji/JARID2 by Western Blot.
Western blot shows lysates of IMR-32 human neuroblastoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Jumonji/JARID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6090) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Jumonji/JARID2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Jumonji/JARID2 Antibody
Western Blot
Sample: IMR‑32 human neuroblastoma cell line and Neuro‑2A mouse neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Jumonji/JARID2
Alternate Names
Gene Symbol
UniProt
Additional Jumonji/JARID2 Products
Product Documents for Jumonji/JARID2 Antibody
Certificate of Analysis
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Product Specific Notices for Jumonji/JARID2 Antibody
For research use only
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Citations for Jumonji/JARID2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting Guide
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