Human/Mouse MCPIP1 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB7875S Datasheet / COA / SDS

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FAB7875S-100UG

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Human/Mouse MCPIP1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human MCPIP1 in direct ELISAs and human and mouse MCPIP1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human MCPIP3 is observed.

Source

Monoclonal Mouse IgG2a Clone # 604421

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human MCPIP1
Asp426-Glu599
Accession # Q5D1E8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MCPIP1

Human MCP-induced protein (MCPIP), also known as ZC3H12A, is an approximately 66 kDa intracellular protein that contains one RNAse domain (aa 134-290), one zinc finger (aa 301-324), and a proline-rich region (aa 458-536). Within aa 426-599, human MCPIP shares approximately 79% aa sequence identity with mouse and rat MCPIP. Its expression is induced by inflammatory stimulation and cellular stress. It acts to dampen inflammatory responses by promoting the degradation of proinflammatory cytokine mRNAs, inhibiting NFkB activation, and antagonizing TLR signaling. MCPIP exhibits deubiquitinase activity and inhibits the biogenesis of miRNA. It also enhances inflammation-induced angiogenesis, osteocyte and adipocyte differentiation, and the cell death of cardiac myocytes.

Long Name

MCP-1-induced Protein 1

Entrez Gene IDs

80149 (Human); 230738 (Mouse); 313587 (Rat)

Alternate Names

MCPIP1; Reg1; Regnase-1; ZC3H12A; zinc finger CCCH-type containing 12A