Human/Mouse NPM1 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF5205S Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5205S-100UG

Save 15% on Select RUO Reagents. See Details.

All NPM1 Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NPM1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse NPM1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NPM1
Met9-Leu158
Accession # P06748

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NPM1

NPM1 (Nucleophosmin 1; also nucleolar protein 23) is a 40 kDa member of the nucleoplasmin family of proteins. It is found in the nucleus where it performs multiple functions. NPM1 normally associates with unduplicated centrosomes. After phosphorylation at Thr199, it dissociates from centrosomes, allowing for centrosome duplication. NPM1 also negatively regulates GCN5-dependent histone acetylation during mitosis. Human NPM1 is 294 amino acids (aa) in length. It contains a Met-rich region (aa 1‑9), two acidic regions (aa 120‑132 and 161‑188) and two NLSs (aa 152‑157 and 191‑197). Phosphorylation occurs at Ser4/10/70/254. Splice variants show a deletion of aa 195‑223, a two aa substitution for aa 258‑294 and an 11 aa substitution for aa 288‑294. Over aa 9‑158, human NPM1 is 97% aa identical to mouse NPM1.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Entrez Gene IDs

4869 (Human); 434373 (Mouse); 300303 (Rat)

Alternate Names

B23; MGC104254; NPM1; NPMnucleophosmin/nucleoplasmin family, member 1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Nucleophosmin; Numatrin