NRARP (Notch-regulated ankyrin repeat protein) is a 13 kDa (predicted) protein that is induced by Notch activation. In SDS-PAGE, this protein migrates at 25‑30 kDa. It is expressed in multiple cell types such as fibroblasts and thymocyte precursors. NRARP is presumed to block Notch1-mediated conversion of the CSL transcription factor from a transcriptional suppressor to activator. It is also a positive regulator of the Wnt pathway by stabilizing LEF1. Human NRARP is 114 amino acids (aa) in length. It contains two C-terminal ankyrin-like repeats (aa 50‑82 and 83‑114) that may be involved in protein-protein interactions. Human and mouse NRARP are identical in amino acid sequence.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Discontinued Product
AF4814 has been discontinued.
View all NRARP products.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human NRARP
Ser2-Arg114
Accession # Q7Z6K4
Ser2-Arg114
Accession # Q7Z6K4
Specificity
Detects human and mouse NRARP in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NRARP Antibody
Detection of Human/Mouse NRARP by Western Blot.
Western blot shows lysates of human placenta, liver, and kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NRARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4814) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NRARP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for NRARP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human placenta, liver and kidney tissue
Sample: Human placenta, liver and kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: NRARP
Long Name
Notch-Regulated Ankyrin Repeat Protein
Alternate Names
MGC61598, NOTCH-regulated ankyrin repeat protein, notch-regulated ankyrin repeat-containing protein
Gene Symbol
NRARP
UniProt
Additional NRARP Products
Product Documents for NRARP Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for NRARP Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
