Patched 1/PTCH (First Extracellular Loop) Antibody (413220)

R&D Systems | Catalog # MAB41051

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 413220
View all Patched 1/PTCH Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Patched 1/PTCH
Glu108-Asp422
Accession # Q61115

Specificity

Detects mouse Patched 1/PTCH (First Extracellular Loop) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 15% cross‑reactivity with recombinant human Patched 2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Patched 1/PTCH (First Extracellular Loop) Antibody (413220)

Detection of Mouse Patched 1/PTCH antibody by Western Blot.

Detection of Mouse Patched 1/PTCH by Western Blot.

Western blot shows lysates of MEF mouse embryonic feeder cells, Neuro-2A mouse neuroblastoma cell line, and mouse brain tissue. PVDF Membrane was probed with 3 µg/mL of Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Patched 1/PTCH at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Patched 1/PTCH (First Extracellular Loop) Antibody (413220)

Application
Recommended Usage

Western Blot

3 µg/mL
Sample: MEF mouse embryonic feeder cells, Neuro‑2A mouse neuroblastoma cell line, and mouse brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Patched 1/PTCH

Patched homolog 1 (PTCH, also called PTC, PTC1 or PTCH1) is a 170‑175 kDa member of the Patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to PTC1, Smoothened is released and initiates downstream signaling. Mouse PTCH is a 12 transmembrane, 1434 aa glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for PTCH has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse PTCH shares 97% and 99% aa sequence identity with human and rat PTCH, respectively.

Alternate Names

BCNS, HPE7, mes, NBCCS, ptc1, PTCH1

Entrez Gene IDs

5727 (Human); 19206 (Mouse); 89830 (Rat)

Gene Symbol

PTCH1

UniProt

Q61115

Additional Patched 1/PTCH Products

Product Documents for Patched 1/PTCH (First Extracellular Loop) Antibody (413220)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Patched 1/PTCH (First Extracellular Loop) Antibody (413220)

For research use only

Citations for Patched 1/PTCH (First Extracellular Loop) Antibody (413220)

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