Human/Mouse PKN1 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB6100S Datasheet / COA / SDS

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FAB6100S-100UG

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Human/Mouse PKN1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse PKN1 in Western blots.

Source

Monoclonal Mouse IgG1 Clone # 540421

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PKN1
Ile215-Thr388
Accession # Q16512

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PKN1

PKN1 (serine/threonine protein kinase N1), also known as PKN or PRK, is a ubiquitous 120 kDa cytoplasmic kinase of the PKC superfamily. The 942 amino acid (aa) PKN1 contains three HR1 repeats (aa 34‑263), a C2 domain (aa 325‑461), and a protein kinase domain (aa 615‑874). PKN1 can be activated by proteolysis, lipid interaction, or Rho G proteins, and is a downstream effector of RhoA. It promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. In vivo, PKN1 phosphorylates histone H3 at the Thr11 position.

Long Name

Serine/Threonine Protein Kinase N1

Entrez Gene IDs

5585 (Human); 320795 (Mouse); 29355 (Rat)

Alternate Names

DBK; EC 2.7.11; MGC46204; PAK1; PAK1Serine-threonine protein kinase N; PKN; PKN1; PKN-ALPHA; PKNPKN-ALPHA; PRK1; PRK1PAK-1; PRKCL1; PRKCL1protein kinase C-related kinase 1; Protease-activated kinase 1; Protein kinase C-like 1EC 2.7.11.13; Protein kinase C-like PKN; protein kinase N1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine/threonine protein kinase N; serine/threonine-protein kinase N1; serine-threonine kinase N