PSMB7 Antibody (787807)

R&D Systems | Catalog # MAB7590

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 787807
View all PSMB7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PSMB7
Thr44-Ser277
Accession # Q99436

Specificity

Detects human PSMB7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PSMB10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PSMB7 Antibody (787807)

Detection of Human and Mouse PSMB7 antibody by Western Blot.

Detection of Human and Mouse PSMB7 by Western Blot.

Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, beta TC-6 mouse beta cell insulinoma cell line, AR42J human acinar pancreatic cancer cell line, HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, MCF-7 human breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human PSMB7 Monoclonal Antibody (Catalog # MAB7590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PSMB7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PSMB7 Antibody (787807)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: 786‑O human renal cell adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PSMB7

PSMB7 (proteasome subunit, beta-type 7), also called proteasome subunit Z, is a widely expressed, non-catalytic component of the 20S proteasome core within the 26S proteasome that stabilizes late stages of proteasome assembly. Human PSMB7 is synthesized as a 277 amino acid (aa) protein with a 43 aa propeptide and a 234 active protein that contains one potential tyrosine phosphorylation site. Active human PSMB7 shares 97% aa sequence identity with mouse and rat PSMB7. Total PSMB7 expression is positively correlated with chemotherapy resistance in human breast cancer.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 7

Alternate Names

Macropain Chain Z, Proteasome Subunit Z

Entrez Gene IDs

5695 (Human); 19177 (Mouse); 85492 (Rat)

Gene Symbol

PSMB7

UniProt

Q99436 (Human)

Additional PSMB7 Products

Product Documents for PSMB7 Antibody (787807)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PSMB7 Antibody (787807)

For research use only

Related Research Areas

Cancer Biomarkers

Citations for PSMB7 Antibody (787807)

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