Human/Mouse/Rat 14-3-3 gamma Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB5700X Datasheet / COA / SDS

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FAB5700X-100UG

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All 14-3-3 gamma Antibodies

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Human/Mouse/Rat 14-3-3 gamma Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat 14-3-3 gamma in Western blots.

Source

Monoclonal Mouse IgG2b Clone # 505810

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human 14-3-3Î³
Met1-Asn247
Accession # P61981

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 14-3-3 gamma

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ. 14-3-3 gamma becomes phosphorylated in response to PDGF and subsequently interacts with different PKC isoforms.

Entrez Gene IDs

7532 (Human); 22628 (Mouse); 56010 (Rat)

Alternate Names

1433 gamma; 14-3-3 gamma; 14-3-3 protein gamma; 14-3-3GAMMA; KCIP-1; Protein kinase C inhibitor protein 1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide; YWHAG