Human/Mouse/Rat alpha -Actinin 1 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB8279T Datasheet / COA / SDS

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FAB8279T-100UG

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Human/Mouse/Rat alpha -Actinin 1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human alpha -Actinin 1 in ELISA, and human, mouse and rat alpha -Actinin 1 in Western Blot.

Source

Monoclonal Mouse IgG2b Clone # 910301

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Î±-Actinin 1
Asn272-Thr500
Accession # P12814

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: alpha Actinin 1

alpha -Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa. It is 892 amino acids (aa) in length and shares 99% aa identity with mouse and rat ACTN1. Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns. ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Long Name

Actinin alpha 1

Entrez Gene IDs

87 (Human); 109711 (Mouse); 81634 (Rat)

Alternate Names

actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha Actinin 1; alphaActinin 1; alpha-Actinin 1; Alpha-actinin cytoskeletal isoform; alpha-actinin-1; BDPLT15; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1