Human/Mouse/Rat AlphaB Crystallin/CRYAB Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB4849N Datasheet / COA / SDS

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FAB4849N-100UG

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Human/Mouse/Rat AlphaB Crystallin/CRYAB Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human AlphaB Crystallin/CRYAB in direct ELISAs and human, mouse, and rat AlphaB Crystallin/CRYAB in Western blots. In direct ELISAs, no cross-reactivity with recombinant human AlphaA Crystallin/CRYAA is observed.

Source

Monoclonal Mouse IgG2b Clone # 731502

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Crystallin/CRYAB
Met1-Lys175
Accession # P02511

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AlphaB Crystallin/CRYAB

CRY-alpha B (crystalline alpha -B chain/CRYAB; also HspB5) is a 22-23 kDa member of the HSP20 family of proteins. It has widespread expression, and is found in lens epithelium where it noncovalently oligomerizes with CRYAA to generate a transparent 350-1000 kDa alpha -crystalline protein complex. Human CRYAB is 175 amino acids (aa) in length. It contains an alpha -crystalline Hsp domain over aa 66-149. Multiple post-translational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. It may also be phosphorylated at Ser45 and 59, be potentially O-GlcNAc modified at Thr158, 162 or 170, and acetylated at Lys92. An alternate start site at Met68 may be accompanied by a 47 aa substitution for aa 109-175. Full-length human CRYAB shares 54% aa sequence identity with CRYAA, and 98% aa identity with mouse CRYAB.

Entrez Gene IDs

1410 (Human); 12955 (Mouse); 25420 (Rat)

Alternate Names

alpha B crystallin; alpha(B)-crystallin; AlphaB Crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component