Human/Mouse/Rat ARK5/NUAK1 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB6978N Datasheet / COA / SDS

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FAB6978N-100UG

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All ARK5/NUAK1 Antibodies

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Human/Mouse/Rat ARK5/NUAK1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human ARK5/NUAK1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SNARK is observed.

Source

Monoclonal Mouse IgG1 Clone # 715901

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human ARK5/NUAK1
Lys311-Met462
Accession # O60285

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ARK5/NUAK1

AMPK-related kinase 5 (ARK5), also known as NUAK1, is a 74 kDa intracellular Ser/Thr kinase that promotes cell survival during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5 overexpression in several cancers contributes to increased tumor progression and aggressiveness. ARK5 contains one catalytic domain (aa 65-306), and a short isoform of the human protein is prematurely tuncated within this domain. Within aa 311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and rat ARK5, respectively.

Long Name

AMPK-related Protein Kinase 5

Entrez Gene IDs

9891 (Human); 77976 (Mouse); 299694 (Rat)

Alternate Names

AMPK-related protein kinase 5; ARK5; ARK5AMP-activated protein kinase family member 5; EC 2.7.11; EC 2.7.11.1; KIAA0537NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; NUAK1