Blk Antibody

R&D Systems | Catalog # AF2679

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Blk Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Blk
Met1-Val63
Accession # P51451

Specificity

Detects human, mouse, and rat Blk in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Blk Antibody

Detection of Human/Mouse/Rat Blk antibody by Western Blot.

Detection of Human/Mouse/Rat Blk by Western Blot.

Western blot shows lysates of A20 mouse B cell lymphoma cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Blk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2679) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Blk at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Blk Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: A20 mouse B cell lymphoma cell line and CH-1 mouse B cell lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Blk

B-cell lymphocyte kinase (Blk) is a member of the Src family of non‑receptor tyrosine kinases. Constitutively active Blk induces malignant transformation of B-cell progenitors, suggesting a role for Blk in B-lineage cell differentiation and proliferation.

Long Name

B Lymphoid Tyrosine Kinase

Alternate Names

B lymphocyte kinase, B lymphoid tyrosine kinase, BLK nonreceptor tyrosine kinase, EC 2.7.10.2, MGC10442, MODY11, p55-Blk, tyrosine-protein kinase Blk

Entrez Gene IDs

640 (Human); 12124 (Mouse); 364403 (Rat)

Gene Symbol

BLK

UniProt

P51451

Additional Blk Products

Product Documents for Blk Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Blk Antibody

For research use only

Related Research Areas

B Cell Activation
Intracellular Kinases

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