Human/Mouse/Rat BOK Alexa Fluor® 700-conjugated Antibody

Catalog #: AF6067N Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6067N-100UG

Save 15% on Select RUO Reagents. See Details.

All BOK Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat BOK Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat BOK in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BOK
Met15-Val123
Accession # Q9UMX3

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BOK

BOK (Bcl-2-related ovarian killer protein; also Bcl2L9 and Mtd) is a 27 kDa member of the Bax subgroup of the Bcl-2 family of molecules. It is expressed in spermatocytes, ovarian granulosa cells, and liver cells. BOK is activated by E2F proteins, and sensitizes replicating cells to apoptosis when under stress. It is normally found in the cytosol, and upon activation, becomes incorporated into mitochondrial membranes. Human BOK is 212 amino acids (aa) in length and contains one BH4 domain (aa 32‑44), one BH3 domain (aa 66‑82), a BH1 domain (aa 112‑131) and a BH2 domain (aa 164‑178). A potential transmembrane span has been identified between aa 189‑210. BOK heterodimerizes with Mcl-1, BHRF-1, Bfl-1 and Bnip3, and is known to form heterooligomers. There are two potential splice variants. One contains an alternative start site at Met145, while another shows a five aa substitution for aa 117‑212. Over aa 15‑123, human BOK shares 94% aa identity with mouse BOK.

Long Name

Bcl-2-related Ovarian Killer

Entrez Gene IDs

666 (Human); 29884 (Rat)

Alternate Names

bcl2-L-9; BCL2L9bcl-2-related ovarian killer protein; Bcl-2-like protein 9; BCL2-related ovarian killer; BOK; BOKL; hBOK; MGC4631