Human/Mouse/Rat Calcineurin B Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Calcineurin B

Background

Calcineurin, also called Protein Phosphatase 2B,

PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is

an enzyme that dephosphorylates serine and

threonine residues in proteins. It is a heterodimer of

a 59,000 dalton catalytic A subunit and a

1,900 dalton regulatory B subunit that is activated

by the binding of calcium ions and calmodulin (1).

Calcineurin is expressed in many tissues, but its

levels are highest in the brain, where it may play a

role in learning and memory (2). It has many

substrates, including NFAT, a transcription factor

that is activated by dephosphorylation (3).

Complexes of the immunosuppressants cyclosporin

and FK506 with immunophilin proteins such as

cyclophilin and FKBP12 are potent and specific

inhibitors of Calcineurin activity (4). Alterations in

Calcineurin activity are suspected to play a role in

cardiac hypertrophy (5) and graft versus host

disease in organ transplantation (6).

1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.

2. Zeng H. et al. (2001) Cell 107:617.

3. Okamura, H. et al. (2000) Molecular Cell 6:539.

4. Liu, J. et al. (1992) Biochemistry 31:3896.

5. Molkentin, J.D. (2000) Circulation Research 87:731.

6. Sanquer, S.A. et al. (2004) Transplantation 77:854.

Product Datasheets

Related Research Areas

• Calcium-binding Proteins and Related Molecules
• C-type Lectin Receptors
• Epigenetics
• Intracellular Calcium Signaling in VSMC
• Neurofibrillary Tangle Formation
• Phosphatases and Regulators
• Phosphatases in the Akt Pathway
• Search Kinases and Phosphatases
• Wnt Intracellular Signaling