CARD11/CARMA1 Antibody

R&D Systems | Catalog # AF4839

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CARD11/CARMA1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CARD11
Lys263-Ser442
Accession # Q9BXL7

Specificity

Detects endogenous human, mouse, and rat CARMA1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CARD11/CARMA1 Antibody

Detection of Human/Mouse/Rat CARD11/CARMA1 antibody by Western Blot.

Detection of Human/Mouse/Rat CARD11/CARMA1 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CARD11/CARMA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4839) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CARD11/CARMA1 at approximately 133 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for CARD11/CARMA1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CARD11/CARMA1

CARMA1 (CARD-MAGUK1; also CARD11) is a 133 kDa member of the MAGUK family of proteins. It is a cytoplasmic promoter of Bcl10 phosphorylation, and as such, regulates the effectiveness of NF-kappa B signaling. Human CARMA1 is 1154 amino acids (aa) in length and contains an N-terminal CARD region, two coiled-coil (CC) domains (aa 123‑250 and 295‑442), and a PZD, SH3 and MAGUK domain (aa 996‑1133). The CC1 and MAGUK domains position the molecule, while CC1 and CC2 mediate a necessary dimerization/oligomerization. There is one potential alternate start site seven aa upstream from the standard start site. Over aa 263-442, human CARMA1 is 98% aa identical to both mouse and canine CARMA1.

Long Name

Caspase Recruitment Domain-containing Protein 11

Alternate Names

BIMP3, CARMA1

Entrez Gene IDs

84433 (Human); 108723 (Mouse)

Gene Symbol

CARD11

UniProt

Q9BXL7 (Human)

Additional CARD11/CARMA1 Products

Product Documents for CARD11/CARMA1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for CARD11/CARMA1 Antibody

For research use only

Citations for CARD11/CARMA1 Antibody

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