Chk1 Antibody

R&D Systems | Catalog # AF1630

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Chk1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Chk1
Tyr157-Thr476
Accession # O14757

Specificity

Detects human, mouse, and rat Chk1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Chk1 Antibody

Detection of Human, Mouse, and Rat Chk1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Chk1 by Western Blot.

Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, CEM human T-lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Chk1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1630) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Chk1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Chk1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 µg/500 µg cell lysate
Sample: U2OS human osteosarcoma cell line and Balb/3T3 mouse embryonic fibroblast cell line, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, CEM human T-lymphoblastoid cell line, and Balb/3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chk1

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic stress, Chk1 is phosphorylated by ATM or ATM-related kinases. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Entrez Gene IDs

1111 (Human); 12649 (Mouse); 140583 (Rat)

Gene Symbol

CHEK1

UniProt

O14757

Additional Chk1 Products

Product Documents for Chk1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Chk1 Antibody

For research use only

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