CLEC-2/CLEC1B Antibody

R&D Systems | Catalog # AF1718

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Immunoprecipitation, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CLEC-2/CLEC1B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC-2/CLEC1B
Gln58-Pro229
Accession # AAF36777

Specificity

Detects human, mouse, and rat CLEC-2/CLEC1B in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CLEC-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CLEC-2/CLEC1B Antibody

Detection of Mouse and Rat CLEC-2/CLEC1B antibody by Western Blot.

Detection of Mouse and Rat CLEC-2/CLEC1B by Western Blot.

Western blot shows lysates of mouse kidney tissue, rat kidney tissue, and mouse bone marrow. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CLEC-2/CLEC1B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CLEC-2/CLEC1B antibody by Western Blot.

Detection of Human CLEC-2/CLEC1B by Western Blot.

Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CLEC-2/CLEC1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for CLEC-2/CLEC1B at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for CLEC-2/CLEC1B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse kidney tissue, rat kidney tissue, mouse bone marrow, and human platelets

Reviewed Applications

Read 1 review rated 4 using AF1718 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet‑aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

References

  1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
  4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
  6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
  7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  8. Chaipan, C. et al. (2006) J. Virol. 80:8951.

Long Name

C-type Lectin-like Receptor 2

Alternate Names

CLEC1B, CLEC2

Entrez Gene IDs

51266 (Human); 56760 (Mouse)

Gene Symbol

CLEC1B

UniProt

AAF36777

Additional CLEC-2/CLEC1B Products

Product Documents for CLEC-2/CLEC1B Antibody

Certificate of Analysis

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Product Specific Notices for CLEC-2/CLEC1B Antibody

For research use only

Citations for CLEC-2/CLEC1B Antibody

Customer Reviews for CLEC-2/CLEC1B Antibody (1)

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  • Human/Mouse/Rat CLEC-2/CLEC1B Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE
    Species: Human
    Verified Customer | Posted 10/05/2020
    Immunohistochemistry-Paraffin: Image of FFPE human Lymph node stained with Anti-CLEC-2/CLEC-1B antibody [JC/70A] (antigen retrieval at pH=9, dilution 1/100, incubation overnight at 4°C).
    CLEC-2/CLEC1B Antibody AF1718

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