Human/Mouse/Rat CRADD Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4680S Datasheet / COA / SDS

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AF4680S-100UG

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Human/Mouse/Rat CRADD Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat CRADD in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CRADD
Met1-Glu199
Accession # O88843

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CRADD

CRADD (Caspase and RIP adapter with death domain; also RAIDD) is a 22 kDa, widely-expressed cytosolic adaptor protein that participates in apoptosis. Mouse CRADD is 199 amino acids (aa) in length. It contains an N-terminal CARD region (aa 1‑92) and a C-terminal DD/death domain (aa 116‑188). The CARD interacts with caspase-2, while the DD recruits either PIDD or RIP. These interactions contribute to the activation of an apoptotic cascade. Mouse CRADD shows two potential additional isoforms. One shows an alternate start site at Met55, while a second shows a 70 aa substitution for aa’s 101‑199. Mouse CRADD shares 90% and 95% aa identity with human and rat CRADD, respectively.

Long Name

CASP2 and RIPK1 Domain Containing Adaptor With Death Domain

Entrez Gene IDs

8738 (Human); 12905 (Mouse); 314756 (Rat)

Alternate Names

CASP2 and RIPK1 domain containing adaptor with death domain; Caspase and RIP adapter with death domain; caspase and RIP adaptor with death domain; CRADD; death domain containing protein CRADD; death domain-containing protein CRADD; MGC9163; RAIDD; RAIDDdeath adaptor molecule RAIDD; RIP-associated ICH1/CED3-homologous protein with death domain; RIP-associated protein with a death domain