Human/Mouse/Rat CSRP1 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5739U Datasheet / COA / SDS

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AF5739U-100UG

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Human/Mouse/Rat CSRP1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat CSRP1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CSRP1
Met1-Glu193
Accession # P21291

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CSRP1

CSRP1 (Cysteine-rich protein 1; also CRP1 and Cysteine and Glycine‑rich protein 1) is a 23 kDa cytoplasmic member of the CRP family of LIM domain‑containing proteins. It is expressed in both visceral and vascular smooth muscle, and interacts with zyxin and alpha ‑actinin, two proteins involved in actin assembly and crosslinking, during muscle cell differentiation. Human CSRP1 is 193 amino acids (aa) in length and contains two 50 aa LIM (for lin11/isl1/mec3) domains that contain two zinc‑finger motifs (aa 10‑61 and 119‑171), an NLS between aa 64‑69, and a Gly-rich C-terminus (aa 176‑187). There are two likely phosphorylation sites at Tyr127 and Ser192. Full‑length human CSRP1 shares 99% aa identity with mouse CSRP1.

Long Name

Cysteine-rich Protein 1

Entrez Gene IDs

1465 (Human); 13007 (Mouse); 29276 (Rat)

Alternate Names

CRP; CRP1; CSRP1; CSRPCYRP; CYRP; cysteine and glycine-rich protein 1; Cysteine-rich protein 1; D1S181E; DKFZp686M148; LIM-domain protein