Human/Mouse/Rat DGK-theta Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB6598X Datasheet / COA / SDS

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FAB6598X-100UG

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All DGK-theta Antibodies

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Human/Mouse/Rat DGK-theta Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha, -beta, -gamma, -epsilon, -iota, -kappa, or -zeta is observed.

Source

Monoclonal Mouse IgG1 Clone # 652308

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human DGK-Î¸
Asp696-Lys839
Accession # P52824

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DGK-theta

Diacylglycerol kinase theta (DGK-theta ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-theta binds to RhoA, Akt, PKC epsilon and eta, and Steroidogenic Factor. It translocates to the nucleus or to the plasma membrane in response to NGF, TPA, or GPCR stimulation (thrombin, bradykinin, norepinephrine). DGK-theta contains three Cys-rich zinc finger regions (aa 60-108, aa 121-168, and aa 183-234), a Ras-associating domain (aa 395-494), two LxxLL motifs (aa 555-559 and aa 574-578), and a catalytic domain (aa 590-714). Over aa 696-839, human DGK-theta shares 90% aa sequence identity with mouse and rat DGK-theta.

Long Name

Diacylglycerol Kinase, theta 110kDa

Entrez Gene IDs

1609 (Human)

Alternate Names

DAG kinase theta; DAGK; DAGK4EC 2.7.1.107; DAGK7; DGKQ; DGKtheta; DGK-theta; diacylglycerol kinase theta; diacylglycerol kinase, theta (110kD); diacylglycerol kinase, theta 110kDa; Diglyceride kinase theta