FK506 binding protein, 12 kilodalton molecular weight (FKBP12), also called FKBP1, was originally characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. Proline isomerase activity was demonstrated but not used for quality control. The macrolide immunosuppressants FK506 (Tacrolimus) and rapamycin bind to FKBP12 with high affinity, while the structurally related compound cyclosporine binds with a much lower affinity (1). The binding of these drugs causes FKBP12 to become a potent inhibitor of calcineurin phosphatase activity (2) and TOR kinase activity (3). The inhibition of protein phosphatase activity is highly selective for calcineurin (2), making the FK506/FKBP12 complex a useful tool in the study of this enzyme. Knockout mice lacking FKBP12 are morphologically normal, but develop cardiomyopathies that may be related to dysregulation of ryanodyne receptors (4).
Human/Mouse/Rat FKBP12 Alexa Fluor™ Plus 405‑conjugated Antibody
R&D Systems | Catalog # FAB3777AFP405
Key Product Details
Species Reactivity
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Applications for Human/Mouse/Rat FKBP12 Alexa Fluor™ Plus 405‑conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Formulation
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Stability & Storage
Background: FKBP12
References
- Hamilton, G.S. and J.P. Steiner (1998) J. Med. Chem. 41:5119.
- Liu, J. et al. (1992) Biochemistry 31:3896.
- Toral-Barza, L. et al. (2005) Biochem. Biophys. Res. Comm. 332:304.
- Hamilton, S.L and M.M. Matzuk (1998) Nature 391:489.
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This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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