Glyoxalase II Antibody

R&D Systems | Catalog # AF5944

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Glyoxalase II Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glyoxalase II
Met49-Ile254
Accession # Q16775

Specificity

Detects human, mouse, and rat Glyoxalase II in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Glyoxalase I is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glyoxalase II Antibody

Detection of Human, Mouse, and Rat Glyoxalase II antibody by Western Blot.

Detection of Human, Mouse, and Rat Glyoxalase II by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, human liver, human kidney, mouse liver, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glyoxalase II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Glyoxalase II at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Glyoxalase II Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, human liver, human kidney, mouse liver, and rat liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glyoxalase II

Glyoxalase II (GLO2; also hydroxyacylglutathione hydrolase 2 and Glx II) is a monomeric, cytosolic 29 kDa member of the Glyoxylase II family, metallo-beta -lactamase superfamily of enzymes. It is expressed in liver and kidney, and converts GLO1-generated lactoylglutathione into lactate and GSH. It is up‑regulated by p63 and p73, and thus serves as a p53-associated survival factor. Human GLO2 is 260 amino acids (aa) in length. It binds two atoms of Zn and contains one substrate binding region (aa 143‑145 plus 249‑252). The 29 kDa form represents 80‑90% of cellular GLO2. There is also a 32‑33 kDa, 308 aa mitochondrial isoform that arises via the use of an alternative start site. Its function is unclear, given that mitochondria do not contain GLO1. There is an additional potential isoform that shows an insertion of nine aa after Thr167. Human GLO2 shares approximately 90% aa identity with mouse GLO2.

Alternate Names

GLX2, HAGH

Entrez Gene IDs

3029 (Human); 14651 (Mouse); 24439 (Rat)

Gene Symbol

HAGH

UniProt

Q16775 (Human)

Additional Glyoxalase II Products

Product Documents for Glyoxalase II Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Glyoxalase II Antibody

For research use only

Related Research Areas

Redox Enzymes

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