GRK1 Antibody (466630)

R&D Systems | Catalog # MAB4498

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 466630
View all GRK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRK1
Leu343-Ser563
Accession # Q15835

Specificity

Detects endogenous human, mouse, and rat GRK1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GRK1 Antibody (466630)

Detection of Human/Mouse/Rat GRK1 antibody by Western Blot.

Detection of Human/Mouse/Rat GRK1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRK1 Monoclonal Antibody (Catalog # MAB4498) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRK1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for GRK1 Antibody (466630)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRK1

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK1 and GRK7 are members of the rhodopsin kinase or visual GRK subfamily.

Long Name

G protein-coupled Receptor Kinase 1

Alternate Names

GPRK1, RHOK

Entrez Gene IDs

6011 (Human); 24013 (Mouse); 81760 (Rat)

Gene Symbol

GRK1

UniProt

Q15835

Additional GRK1 Products

Product Documents for GRK1 Antibody (466630)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for GRK1 Antibody (466630)

For research use only

Related Research Areas

Intracellular Kinases

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