HCLS1 Antibody

R&D Systems | Catalog # AF5789

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HCLS1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HCLS1
Met1-Gly84
Accession # P14317

Specificity

Detects endogenous human, mouse and rat HCLS1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HCLS1 Antibody

Detection of Human/Mouse/Rat HCLS1 antibody by Western Blot.

Detection of Human/Mouse/Rat HCLS1 by Western Blot.

Western blot shows lysates of L1.2 mouse pro-B cell line, Nb2-11 rat lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat HCLS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5789) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HCLS1 at approximately 75 to 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for HCLS1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: L1.2 mouse pro-B cell line, Nb2-11 rat lymphoma cell line, and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HCLS1

HCLS1 (Hematopoietic lineage cell-specific protein; also HS1 and LckBP1) is a cytoplasmic substrate for non‑receptor type protein‑tyrosine kinases. Although its predicted MW is 54 kDa, it migrates anomalously at 75‑80 kDa in SDS-PAGE. HCLS1 is restricted to myeloid/lymphoid lineage cell types, including platelets, B cells, T cells and mast cells. HCLS1 binds to F-actin and promotes actin polymerization. It also serves as an intermediate for src-mediated signal transduction. Human HCLS1 is 486 amino acids (aa) in length. It contains one HAX1 binding site (aa 27‑66), three-plus cortactin type repeats (aa 79‑212), a coiled‑coil region (aa 215‑280) and an SH3 domain (aa 437‑484). HCLS1 is initially phosphorylated by Syk at Tyr378 and Tyr397, followed by a Src family kinase phosphorylation at Tyr222. This activates HCLS1, allowing it to interact with Ras-associated Grb2.

Long Name

Hematopoietic Cell-specific Lyn Substrate 1

Alternate Names

Cortactin-like, CTTNL, LckBP1

Entrez Gene IDs

3059 (Human); 15163 (Mouse); 288077 (Rat)

Gene Symbol

HCLS1

UniProt

P14317

Additional HCLS1 Products

Product Documents for HCLS1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for HCLS1 Antibody

For research use only

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