Human/Mouse/Rat HSP60 MAb (Clone 264233)

R&D Systems | Catalog # A-420

Product is in the process of being discontinued.
R&D Systems
Discontinued Product
A-420 has been discontinued. View all HSP60 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 264233
View all HSP60 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSP60
Met1-Phe573

Specificity

Detects human, mouse and rat HSP60 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat HSP60 MAb (Clone 264233)

Detection of Human HSP60 antibody by Western Blot.

Detection of Human HSP60 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP60 Monoclonal Antibody (Catalog # A-420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP60 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat HSP60 MAb (Clone 264233)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: HSP60

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat shock or other cellular stress.

Long Name

Heat Shock Protein 60

Alternate Names

cpn60, GROEL, HSPD1, SPG13

Entrez Gene IDs

3329 (Human); 110907 (Mouse); 63868 (Rat)

Gene Symbol

HSPD1

Additional HSP60 Products

Product Documents for Human/Mouse/Rat HSP60 MAb (Clone 264233)

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat HSP60 MAb (Clone 264233)

For research use only

Related Research Areas

Heat Shock Proteins (HSPs)

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