Human/Mouse/Rat IKK gamma Antibody
Human/Mouse/Rat IKK gamma Antibody Summary
Accession # O88522
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse and Rat IKK gamma by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for IKK gamma at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK gamma
I kappa B kinase gamma (IKK gamma ) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex.
Citations for Human/Mouse/Rat IKK gamma Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Non-catalytic ubiquitin binding by A20 prevents psoriatic arthritis-like disease and inflammation
Authors: B Razani, MI Whang, FS Kim, MC Nakamura, X Sun, R Advincula, JA Turnbaugh, M Pendse, P Tanbun, P Achacoso, PJ Turnbaugh, BA Malynn, A Ma
Nat. Immunol., 2020-03-16;21(4):422-433.
Species: Mouse, Transgenic Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
Expression of type III interferon (IFN) in the vaginal mucosa is mediated primarily by dendritic cells and displays stronger dependence on NF-kappaB than type I IFNs.
Authors: Iversen MB, Ank N, Melchjorsen J, Paludan SR
J. Virol., 2010-02-24;84(9):4579-86.
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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