Human/Mouse/Rat IKK gamma Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of Mouse and Rat IKK gamma  by Western Blot.
1 Image
Product Details
Citations (2)
Supplemental Products

Human/Mouse/Rat IKK gamma Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse, and rat IKK gamma in Western blot.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse IKK gamma
Accession # O88522
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat IKK? antibody by Western Blot. View Larger

Detection of Mouse and Rat IKK gamma by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for IKK gamma at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK gamma

I kappa B kinase gamma (IKK gamma ) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex.

Long Name
IkB Kinase gamma
Entrez Gene IDs
8517 (Human); 309295 (Rat)
Alternate Names
FIP-3IP2; FIP3NF-kappa-B essential modifier; FIP3P; I-kappa-B kinase subunit gamma; IkB kinase gamma subunit; IkB kinase subunit gamma; IkB kinase-associated protein 1; IKBKG; IKK gamma; IKKAP1; IKKG; IKK-gammaIPD2; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IP; IP1; NEMO; NEMOAMCBX1; NFkappaB essential modulator; NF-kappa-B essential modulator

Product Datasheets

You must select a language.


Citations for Human/Mouse/Rat IKK gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Non-catalytic ubiquitin binding by A20 prevents psoriatic arthritis-like disease and inflammation
    Authors: B Razani, MI Whang, FS Kim, MC Nakamura, X Sun, R Advincula, JA Turnbaugh, M Pendse, P Tanbun, P Achacoso, PJ Turnbaugh, BA Malynn, A Ma
    Nat. Immunol., 2020-03-16;21(4):422-433.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  2. Expression of type III interferon (IFN) in the vaginal mucosa is mediated primarily by dendritic cells and displays stronger dependence on NF-kappaB than type I IFNs.
    Authors: Iversen MB, Ank N, Melchjorsen J, Paludan SR
    J. Virol., 2010-02-24;84(9):4579-86.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse/Rat IKK gamma Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat IKK gamma Antibody and earn rewards!

Have you used Human/Mouse/Rat IKK gamma Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review