Human/Mouse/Rat ILKAP/PP2C delta Alexa Fluor® 700-conjugated Antibody

Catalog #: AF4554N Datasheet / COA / SDS

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AF4554N-100UG

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Human/Mouse/Rat ILKAP/PP2C delta Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human ILKAP in Western blots. In direct ELISAs, this antibody shows weak cross-reactivity with PP2C alpha (4%), PP2C beta (6%), and PP2C gamma (1%).

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ILKAP
Met1-His392
Accession # Q9H0C8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ILKAP/PP2C delta

Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a serine/threonine phosphatase that is stimulated by Mn²⁺ ions but inhibited by Mg²⁺and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.

Long Name

Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C

Entrez Gene IDs

80895 (Human); 67444 (Mouse); 64538 (Rat)

Alternate Names

DKFZp434J2031; EC 3.1.3.16; FLJ10181; ILKAP; integrin-linked kinase-associated serine/threonine phosphatase 2C; integrin-linked kinase-associated serine/threonine phosphatase; MGC4846; PP2C delta; PP2C-DELTA; protein phosphatase 2c, delta isozyme