JAB1 Antibody

R&D Systems | Catalog # AF6390

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all JAB1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human JAB1
aa 158-334
Accession # Q92905

Specificity

Detects human, mouse, and rat JAB1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for JAB1 Antibody

Detection of Human, Mouse, and Rat JAB1 antibody by Western Blot.

Detection of Human, Mouse, and Rat JAB1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat JAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6390) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for JAB1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for JAB1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: JAB1

JAB1 (Jun activation domain binding protein-1), also called COPS5 or CSN5 (COP9 signalosome-5), is a 334 amino acid (aa), 38 kDa protein that is a subunit of the COP9 signalosome complex. It mediates cytosolic translocation of the cell cycle inhibitory protein, CDKN1B/p27Kip1, for degradation, thus promoting proliferation. It is widely expressed, especially in proliferating cells and up-regulates granulopoiesis when overexpressed. Within the region used as an immunogen, human JAB1 shares 99% aa identity with mouse and rat JAB1. A 379 aa isoform contains 93 alternate aa in place of the N-terminal 48 aa of the 334 aa form.

Long Name

Jun Activation Domain-binding Protein 1

Alternate Names

COPS5, CSN5, MOV-34, SGN5

Entrez Gene IDs

10987 (Human); 26754 (Mouse); 312916 (Rat)

Gene Symbol

COPS5

UniProt

Q92905 (Human)

Additional JAB1 Products

Product Documents for JAB1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for JAB1 Antibody

For research use only

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