LMW-PTP (Low Molecular Weight Protein Tyrosine Phosphatase; also Red cell acid phosphatase 1/ACP1, HCPTP, and HAAP/Adipocyte acid phosphatase) is a 17‑18 kDa member of the LMWPTP family, PTP superfamily of enzymes. It is widely expressed, being found in cells diverse as B and T cells, endothelial cells and vascular smooth muscle cells. LMW-PTP dephosphorylates multiple substrates, often in a cell-dependent manner. Among the substrates for LMW-PTP include PDGR beta, EphA2 and beta -catenin. PMW-PTP is found intracellularly, generally associated with two cytoplasmic pools, one in the cytosol and the other accompaning the cytoskeleton. Human LMW-PTP is 158 amino acids (aa) in length. The catalytic region spans aa 9-154, and there are two acetylation sites at Ala2 and Lys156. Phosphorylation on Tyr132 and Tyr133 occurs on cytoskeleton-associated LMW-PTP, and has the effect of increasing its phosphatase activity. There are three alternative splice variants reported for LMW-PTP. One shows a 34 aa substitution for aa 41-74, a second shows a deletion of aa 41-74, while a third contains a 35 aa substitution for aa 78-158. The last two isoforms may function a negative regulators of LMW-PTP activity. Full-length LMW-PTP shares 86% aa sequence identity with mouse LMW-PTP.
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala2-His158
Accession # P24666
Specificity
Clonality
Host
Isotype
Scientific Data Images for LMW-PTP/ACP1 Antibody
Detection of Human, Mouse, and Rat LMW‑PTP/ACP1 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, human placenta tissue, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat LMW-PTP/ACP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5075) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMW-PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for LMW-PTP/ACP1 Antibody
Western Blot
Sample: SH‑SY5Y human neuroblastoma cell line, human placenta tissue, TF‑1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using AF5075 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LMW-PTP/ACP1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional LMW-PTP/ACP1 Products
Product Documents for LMW-PTP/ACP1 Antibody
Certificate of Analysis
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Product Specific Notices for LMW-PTP/ACP1 Antibody
For research use only
Related Research Areas
Customer Reviews for LMW-PTP/ACP1 Antibody (1)
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Application: Western BlotSample Tested: Liver and Adult kidneySpecies: Human, Mouse and RatVerified Customer | Posted 02/24/2017
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Protocols
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