Human/Mouse/Rat Nicalin Antibody

R&D Systems | Catalog # AF7686

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Nicalin Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Nicalin
Met1-Ala522, predicted
Accession # Q969V3

Specificity

Detects human, mouse, and rat Nicalin in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Nicalin Antibody

Detection of Human, Mouse, and Rat Nicalin antibody by Western Blot.

Detection of Human, Mouse, and Rat Nicalin by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, CEM human T-lymphoblastoid cell line, Huh-7 human hepatoma cell line, Neuro-2A mouse neuroblastoma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/ Mouse/Rat Nicalin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7686) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nicalin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Nicalin Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line, CEM human T-lymphoblastoid cell line, Huh‑7 human hepatoma cell line, Neuro‑2A mouse neuroblastoma cell line, and NR8383 rat alveolar macrophage cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nicalin

Nicalin (NICAstrin-Like proteIN) is a 60-62 kDa member of the nicastrin family of proteins. It is an ER-embedded transmembrane protein that is found in skeletal and cardiac muscle, hepatocytes and neurons. Nicalin interacts with 22 kDa TMEM147 and 130 kDa NOMO1 to form a 200-220 kDa complex that antagonizes Nodal signaling. Mature human nicalin is a 521 amino acid (aa) type I transmembrane glycoprotein. It contains a 480 aa luminal/extracellular region (aa 43-522) plus a 20 aa cytoplasmic domain (aa 544-563). The luminal region possesses one likely inactive M28 Zn-peptidase-like domain (aa 130-429). Four potential isoform variants are reported. One shows an alternative start site at Met75, a second contains a seven aa insertion after Gln543, a third contains a 102 aa substitution for aa 162-563, and a fourth shows a deletion of Gln446. Over aa 1-522, human nicalin shares 95% aa sequence identity with mouse nicalin.

Alternate Names

Ncl-1, NCLN, NET59

Entrez Gene IDs

56926 (Human); 103425 (Mouse); 314648 (Rat)

Gene Symbol

NCLN

UniProt

Q969V3 (Human)

Additional Nicalin Products

Product Documents for Human/Mouse/Rat Nicalin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Nicalin Antibody

For research use only

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