Human/Mouse/Rat p130Cas Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5730R Datasheet / COA / SDS

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AF5730R-100UG

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Human/Mouse/Rat p130Cas Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat p130Cas in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human p130Cas
Met66-Val412
Accession # P56945

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: p130Cas

p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105‑130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3‑65), a Pro‑rich region (aa 74‑87), at least 10 phosphotyrosine (aa 128‑410) and four phosphoserine (aa 134‑639) sites, an SH3 binding motif (aa 635‑643) and a HLH dimerization segment that interacts with CASL.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Entrez Gene IDs

9564 (Human); 12927 (Mouse); 25414 (Rat)

Alternate Names

BCAR1; breast cancer estrogen resistance 1; Cas scaffolding protein family member 1; CAS1; CASFLJ45059; CASS1; CASS1CRKASbreast cancer estrogen resistance protein 1; CRKAS; Crk-associated substrate p130Cas; CRK-associated substrate; FLJ12176; p130Cas