PKA C alpha Antibody

R&D Systems | Catalog # AF4268

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PKA C alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKA C alpha
Met1-Phe351
Accession # P17612

Specificity

Detects human, mouse, and rat PKA C alpha in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA C beta or rhPKA C delta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PKA C alpha Antibody

Detection of Human/Mouse/Rat PKA Ca antibody by Western Blot.

Detection of Human/Mouse/Rat PKA C alpha by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Mouse/Rat PKA Ca Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (3 ng/lane) were included. A specific band for PKA C alpha was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for PKA C alpha Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: MCF-7 human breast cancer cell line

Reviewed Applications

Read 1 review rated 5 using AF4268 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKA C alpha

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha

Alternate Names

Cs, Pkaca, PKCA1, PKCD, PRKACA

Entrez Gene IDs

5566 (Human); 18747 (Mouse); 25636 (Rat)

Gene Symbol

PRKACA

UniProt

P17612 (Human)

Additional PKA C alpha Products

Product Documents for PKA C alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PKA C alpha Antibody

For research use only

Citations for PKA C alpha Antibody

Customer Reviews for PKA C alpha Antibody (1)

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  • Human/Mouse/Rat PKA C alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult lung
    Species: Mouse
    Verified Customer | Posted 11/04/2019
    PKA C alpha Antibody AF4268

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