Human/Mouse/Rat PP1 Inhibitor-2 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4719S Datasheet / COA / SDS

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AF4719S-100UG

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Human/Mouse/Rat PP1 Inhibitor-2 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat PP1 Inhibitor-2 in Western blots. In Western blots, no cross-reactivity with recombinant human PP1 Inhibitor-1 or DARPP-32 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PP1 Inhibitor-2
Met1-Ser205
Accession # P41236

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PP1 Inhibitor-2

Protein Phosphatase 1 Inhibitor-2 (PP1 Inhibitor-2), also called PP1 regulatory subunit 2 (PP1R2), I2, and IPP2, binds to PP1 through an RVxF consensus sequence. The complex inactivates PP1, preventing it from dephosphorylating substrates such as glycogen phosphorylase. Unlike PP1 Inhibitor-1, the binding of PP1 Inhibitor-2 is Protein Kinase A phosphorylation-independent. Dissociation of the PP1 Inhibitor-2 complex is enhanced by phosphorylation at T72 by GSK3, ERK, or CDK. Overexpression of PP1 Inhibitor-2 in mouse heart improves cardiac function without changing organ size.

Long Name

Protein Phosphatase 1 Inhibitor 2

Entrez Gene IDs

5504 (Human); 66849 (Mouse); 192361 (Rat)

Alternate Names

IPP2; IPP-2; IPP2phosphatase inhibitor 2; MGC87148; phosphoprotein phosphatase; PP1 Inhibitor2; PP1 Inhibitor-2; PPP1R2; protein phosphatase 1, regulatory (inhibitor) subunit 2; protein phosphatase inhibitor 2