Human/Mouse/Rat PPP2R4 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB5929R Datasheet / COA / SDS

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FAB5929R-100UG

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Human/Mouse/Rat PPP2R4 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat PPP2R4 in Western blots.

Source

Monoclonal Mouse IgG1 Clone # 529245

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PPP2R4
Glu14-Asn323
Accession # Q15257

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PPP2R4

PPP2R4, also known as serine/threonine protein phosphatase 2A regulatory subunit B’, PR53 isoform, and PTPA, is a widely expressed member of the PTPA-type peptidyl prolyl isomerase family. Human PPP2R4 is 358 amino acids (aa) in length. Three splicing variants produce four isoforms for PPP2R4, and isoform 2 is the standard protein. Isoform 1, isoform 3 and isoform 4 have deletions corresponding to aa 73-107, 45-108, and 73-149 in isoform 2, respectively. Human PPP2R4 is 87% aa identical to mouse and rabbit PPP2R4. PPP2R4 reversibly stimulates the variable phosphotyrosyl phosphatase activity of the PP2A core heterodimer in the presence of ATP and Mg²⁺. It has been shown that p53 downregulates the expression of PPP2R4.

Long Name

Serine/threonine Protein Phosphatase 2A Regulatory Subunit B

Entrez Gene IDs

5524 (Human); 110854 (Mouse)

Alternate Names

EC 5.2.1.8; MGC2184; Phosphotyrosyl phosphatase activator; PP2A phosphatase activator; PP2A subunit B' isoform PR53; PP2A; PP2A, subunit B', PR53 isoform; PPP2R4; protein phosphatase 2A activator, regulatory subunit 4; PTPA; regulatory subunit B' (PR 53); serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B'