Human Properdin, also known as Complement factor P (CFP), is a 53 kDa secreted glycoprotein that exists in plasma as dimers, trimers or tetramers. It a positive regulator of the alternate pathway of complement. Properdin binds to and stabilizes the C3- and C5-convertase enzyme complexes and is thought to connect humoral and cellular immunity. Properdin released from activated neutrophils may play a major role in pattern recognition, whereas plasma properdin may primarily stabilize convertase. Properdin deficiency results in higher susceptibility to bacterial infections. Full-length human Properdin shares 77 % sequence identity with mouse Properdin.
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived human Properdin
Asp28-Leu469
Accession # P27918
Asp28-Leu469
Accession # P27918
Specificity
Detects human, mouse, and rat Properdin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Properdin Antibody
Detection of Human, Mouse, and Rat Properdin by Western Blot.
Western blot shows lysates of human serum, mouse serum, and rat serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Properdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8216) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Properdin at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Properdin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human serum, mouse serum, and rat serum
Sample: Human serum, mouse serum, and rat serum
Reviewed Applications
Read 2 reviews rated 4.5 using AF8216 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Properdin
Alternate Names
CFP, Complement Factor P, PFC
Gene Symbol
CFP
UniProt
Additional Properdin Products
Product Documents for Properdin Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Properdin Antibody
For research use only
Related Research Areas
Citations for Properdin Antibody
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Customer Reviews for Properdin Antibody (2)
4.5 out of 5
2 Customer Ratings
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Application: ImmunofluorescenceSample Tested: Mouse kidneySpecies: MouseVerified Customer | Posted 11/27/2018Mouse glomeruli staining
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Application: Western BlotSample Tested: SerumSpecies: HumanVerified Customer | Posted 08/30/2016Need to load 4 ul of serum to get this quality of WB its not working with 1 UL of serum
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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