Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PSMA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PSMA2
Ala2-Ala234
Accession # P25787

Specificity

Detects human, mouse and rat PSMA2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PSMA2 Antibody

Detection of Human, Mouse, and Rat PSMA2 antibody by Western Blot.

Detection of Human, Mouse, and Rat PSMA2 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PSMA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7639) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PSMA2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PSMA2 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: A172 human glioblastoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NRK rat normal kidney cell line

Reviewed Applications

Read 1 review rated 4 using AF7639 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PSMA2

PSMA2 (Proteasome subunit alpha type 2; also proteasome component C3 and Macropain subunit C3) is an intracellular 25-27 kDa member of the peptidase T1A family of molecules. It is ubiquitously expressed, and participates in the formation of the 700 kDa 20S proteasomal complex. This complex contains four stacked rings, forming a barrel. The top and bottom rings are made of seven alpha -subunits, while the two central rings are composed of seven catalytic beta -subunits. The complex degrades 80-90% of all intracellular proteins through both a ubiquitin-dependent and -independent process. PSMA2 may modulate the activity of select beta -subunits. Human PSMA2 is 234 amino acids (aa) in length. It contains at least five utilized phosphorylation sites plus one nitrated tyrosine. Full-length human PSMA2 shares 99% aa sequence identity with mouse PSMA2.

Long Name

Proteasome (Prosome, Macropain) Subunit, alpha Type, 2

Alternate Names

HC3, MU, PMSA2, PSC3

Entrez Gene IDs

5683 (Human); 19166 (Mouse); 29669 (Rat)

Gene Symbol

PSMA2

UniProt

P25787

Additional PSMA2 Products

Product Documents for Human/Mouse/Rat PSMA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PSMA2 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Customer Reviews for Human/Mouse/Rat PSMA2 Antibody (1)

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  • Human/Mouse/Rat PSMA2 Antibody
    Name: Simon Liu
    Application: Immunohistochemistry
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 12/08/2018
    Human/Mouse/Rat PSMA2 Antibody AF7639

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