Human/Mouse/Rat RalA Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB3270U Datasheet / COA / SDS

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FAB3270U-100UG

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Human/Mouse/Rat RalA Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat RalA in Western blots. In Western blots, no cross-reactivity with recombinant human RalB is observed.

Source

Monoclonal Rat IgG2a Clone # 399527

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human RalA
Met1-Leu206
Accession # P11233

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RalA

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name

Ras-like Protein A

Entrez Gene IDs

5898 (Human); 56044 (Mouse); 81757 (Rat)

Alternate Names

HINCONS; RAL; RalA; Rasl1