Human/Mouse/Rat RalA Antibody

Catalog #: AF3270 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3270
AF3270-SP

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Detection of Human/Mouse/Rat RalA by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RalA Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat RalA in Western blots. When immunoblotted, no cross-reactivity with recombinant human (rh) RalB, rhH-Ras, rhN‑Ras or rhK-Ras is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RalA
Met1-Leu206
Accession # P11233

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

20 µg/mL

Exosome Standards (Human Urine) (Catalog # NBP2-49840) and MDA-MB-468 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat RalA by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat RalA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3270) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (1 ng/lane) were included. A specific band for RalA was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human RalA by Simple Western^TM. Simple Western shows lysates of Exosome Standards (Human Urine) (NBP2-49840) and MDA‑MB‑468 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for RalA at approximately 32 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat RalA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3270). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RalA

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name

Ras-like Protein A

Entrez Gene IDs

5898 (Human); 56044 (Mouse); 81757 (Rat)

Alternate Names

HINCONS; RAL; RalA; Rasl1