The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human RalA
Met1-Leu206
Accession # P11233
Met1-Leu206
Accession # P11233
Specificity
Detects human, mouse and rat RalA in Western blots. When immunoblotted, no cross-reactivity with recombinant human (rh) RalB, rhH-Ras, rhN‑Ras or rhK-Ras is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat RalA Antibody
Detection of Human/Mouse/Rat RalA by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat RalA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3270) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (1 ng/lane) were included. A specific band for RalA was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human RalA by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (Human Urine) (NBP2-49840) and MDA‑MB‑468 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for RalA at approximately 32 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat RalA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3270). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.
Applications for Human/Mouse/Rat RalA Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: Exosome Standards (Human Urine) (Catalog # NBP2-49840) and MDA-MB-468 human breast cancer cell line
Sample: Exosome Standards (Human Urine) (Catalog # NBP2-49840) and MDA-MB-468 human breast cancer cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat brain tissue
Sample: HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: RalA
Long Name
Ras-like Protein A
Alternate Names
Rasl1
Gene Symbol
RALA
UniProt
Additional RalA Products
Product Documents for Human/Mouse/Rat RalA Antibody
Product Specific Notices for Human/Mouse/Rat RalA Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars