(40S) Ribosomal protein S6 (rpS6; also Phosphoprotein NP33) is a 32-34 kDa member of the ribsosmal protein S6e family of molecules. It is ubiquitously expressed and involved in the regulation of cell size. Higher eukaryotic ribosomes contain a small (or 40S) and large (or 60S) subunit. The small subunits are composed of one RNA molecule plus more than 30 individual proteins, one of which is rp6S. It is a substrate of both PASKIN and S6 kinase, and when phosphorylated C-terminally, appears to slow the rate of protein synthesis. Notably, the activities of rpS6 are complex, as phosphorylation seems to decrease cell proliferation and glucose utilization on one hand, while increasing cell size on the other. Human ribosomal protein S6 is 249 amino acids (aa) in length. It possesses two acetylated lysines plus six phosphorylated serines. There are three potential splice forms. One contains a four aa substitution for aa 1-46, a second utilizes an alternative start site at Met32, and a third utilizes the same Met32 start site coupled to a substitution of a Gln residue for aa 234-243. Full-length human, mouse and rat rpS6 are identical in their amino acid sequences.
Human/Mouse/Rat Ribosomal Protein S6/RPS6 Alexa Fluor® 350‑conjugated Antibody
R&D Systems | Catalog # AF5436U
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met1-Lys249
Accession # P62753
Specificity
Clonality
Host
Isotype
Applications
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Ribosomal Protein S6/RPS6
Alternate Names
Gene Symbol
UniProt
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Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
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- Western Blot Troubleshooting Guide
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