SEC13 Antibody (1280C)

R&D Systems | Catalog # MAB90552

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1280C
View all SEC13 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SEC13
Gly123-Gln322
Accession # P55735

Specificity

Detects human SEC13 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC13 Antibody (1280C)

Detection of Human, Mouse, and Rat SEC13 antibody by Western Blot.

Detection of Human, Mouse, and Rat SEC13 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/ Mouse/Rat SEC13 Monoclonal Antibody (Catalog # MAB90552) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for SEC13 Antibody (1280C)

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and H4‑II‑E‑C3 rat hepatoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SEC13

Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport.  Presenillin-1, which is implicated in early-onset Alzheimer's disease has been shown to interact with Sec13 vesicles.  Additionally, Sec13 has been shown to be a component of the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the GATOR2 sub-complex of the GATOR complex.

Long Name

Nuclear Pore Complex Protein Nup85

Alternate Names

D3S1231E, Npp-20, SEC13L1, SEC13R

Entrez Gene IDs

6396 (Human); 110379 (Mouse); 297522 (Rat)

Gene Symbol

SEC13

UniProt

P55735

Additional SEC13 Products

Product Documents for SEC13 Antibody (1280C)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SEC13 Antibody (1280C)

For research use only

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