Human/Mouse/Rat STIM1 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB6976V Datasheet / COA / SDS

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FAB6976V-100UG

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Human/Mouse/Rat STIM1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human STIM1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human STIM2 is observed.

Source

Monoclonal Mouse IgG2a Clone # 705111

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human STIM1
Leu23-Thr182
Accession # Q13586

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: STIM1

STIM-1 (stromal interaction molecule 1), previously called GOK, is a 90 kDa type I transmembrane protein of the endoplasmic reticulum (ER). When STIM-1 senses depletion of calcium in the ER via its EF-hand domain (aa 63-98), it interacts with the plasma membrane Ca2+ release-activated Ca2+ (CRAC) channel Orai1, increasing Ca2+ influx. The human STIM-1 extracellular/lumenal domain (aa 23-213) contains an EF hand Ca2+-binding motif and a SAM (sterile a-motif) multimerization domain. A potential isoform is truncated at aa 491. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2). Within the region used as an immunogen, human STIM1 shares 95% amino acid identity with mouse and rat STIM1.

Long Name

Stromal Interaction Molecule 1

Entrez Gene IDs

6786 (Human); 20866 (Mouse); 361618 (Rat)

Alternate Names

GOK; GOKD11S4896E; STIM1; stromal interaction molecule 1