Human/Mouse/Rat UBR5 Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: UBR5

UBR5 (Ubiquitin protein ligase E3 component n-recognin 5; also HYD and EDD) is a 280‑300 kDa member of the HECT E3 family of ubiquitin (Ub) protein ligases. It is ubiquitously expressed, and found in both the cytoplasm and nucleus of cells. In addition to ubiquination, UBR5 is functionally diverse. In the cytoplasm, it interacts with, and stabilizes APC, thereby increasing its concentration. In the nucleus (of smooth muscle cells), it stabilizes myocardin, a transcription factor that activates smooth muscle-specific genes. Perhaps its best known function involves DNA checkpoint activation. Following DNA damage, UBR5 promotes the phosphorylation of CHK2, which arrests the cell cycle at G2/M. Human UBR5 is 2799 amino acids (aa) in length and contains a Ub-associated domain (aa 180‑215), two NLS's (aa 502‑517 and 630‑635), one UBR-type zinc finger region (aa 1177‑1244), a PABC region (aa 2377‑2454) and a HECT domain with a Ub-binding Cys residue (aa 2462‑2799). Over aa 1‑227, human UBR5 shares 98% aa identity with mouse UBR5.

Product Datasheets

Related Research Areas

• E3 Ubiquitin Ligases