Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SCAMP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SCAMP3
Met1-Leu170
Accession # O14828

Specificity

Detects endogenous human and mouse SCAMP3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse SCAMP3 Antibody

Detection of Human/Mouse SCAMP3 antibody by Western Blot.

Detection of Human/Mouse SCAMP3 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and K562 human chronic myelogenous leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse SCAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5344) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCAMP3 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse SCAMP3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line and human K562 human chronic myelogenous leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JEG-3 human epithelial choriocarcinoma cell line

Reviewed Applications

Read 1 review rated 5 using AF5344 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCAMP3

SCAMP3 (Secretory carrier membrane protein 3) is a 38‑40 kDa member of the SCAMP family of proteins. It is expressed in heart and skeletal muscle, plus fat, and functions in post-Golgi recycling and EGFR internalization pathways. Human SCAMP3 is 347 amino acids (aa) in length. It contains a cytoplasmic N-terminus (aa 1‑170) that shows three NPF repeats (binding sites for EH domain proteins), a heptad repeat of aliphatic aa's, and a potential SH3-binding motif. This is followed by four consecutive transmembrane domains (aa 171‑297) plus a C-terminal cytoplasmic region (aa 298‑347). It appears that phosphorylation of SCAMP3 on Tyr41 is a requisite for its interaction with EGFR. There are three potential splice forms. One shows a deletion of aa 23‑48, while two others show a one and 23 aa substitution for aa 131‑144 and 131‑260, respectively. Over aa 1‑170, human and mouse SCAMP3 are 91% aa identical.

Long Name

Secretory Carrier Membrane Protein 3

Alternate Names

PROPIN1

Entrez Gene IDs

10067 (Human); 24045 (Mouse); 65169 (Rat)

Gene Symbol

SCAMP3

UniProt

O14828

Additional SCAMP3 Products

Product Documents for Human/Mouse SCAMP3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SCAMP3 Antibody

For research use only

Citations for Human/Mouse SCAMP3 Antibody

Customer Reviews for Human/Mouse SCAMP3 Antibody (1)

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  • Human/Mouse SCAMP3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 1XTBS 0.05% Tween, 5% milk<br />Dilution: 1:1000

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