Human/Mouse sFRP-3 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB192X Datasheet / COA / SDS

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FAB192X-100UG

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Human/Mouse sFRP-3 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human sFRP-3 in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse (rm) sFRP‑3 and no cross-reactivity with recombinant human (rh) sFRP-1, rmsFRP-2, or rhsFRP-4.

Source

Monoclonal Mouse IgG1 Clone # 133912

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human sFRP-3
Ala33-Asn325
Accession # AAB51298

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: sFRP-3

sFRP-3, also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt signaling.

Long Name

Secreted Frizzled Related Protein 3

Entrez Gene IDs

2487 (Human); 20378 (Mouse)

Alternate Names

FIZ; FREOS1; Frezzled; Fritz; Frizzled-related protein 1; frizzled-related protein; FRP; FRP-3; FRZB; Frzb1; FRZB-1; FRZB1sFRP-3; FRZB-PEN; FZRB; hFIZ; secreted frizzled-related protein 3; sFRP3; sFRP-3; SFRP3frezzled; SRFP3frizzled homolog-related