TIAM1 Antibody

R&D Systems | Catalog # AF5038

R&D Systems
Discontinued Product
AF5038 has been discontinued. View all TIAM1 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TIAM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TIAM1
Met1-Ala378
Accession # Q13009

Specificity

Detects endogenous human and mouse TIAM1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for TIAM1 Antibody

Detection of Human/Mouse TIAM1 antibody by Western Blot.

Detection of Human/Mouse TIAM1 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, HCT-116 human colorectal carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TIAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5038) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIAM1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for TIAM1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: D3 mouse embryonic stem cell line, HCT-116 human colorectal carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line

Reviewed Applications

Read 3 reviews rated 3.7 using AF5038 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIAM1

TIAM1 (T lymphoma invasion and metastasis) is an approximately 200 kDa member of the TIAM family of guanine-nucleotide exchange factors. It is expressed in multiple cell types, including endothelial cells, neurons, Schwann cells and almost all tumors. TIAM1 stimulates the activity of RHO-like GTPases and thus connects extracellular signals to the cytoskeleton. Human TIAM1 is 1591 amino acids (aa) in length. It contains a myristoylation site at Gly2, plus two pleckstrin homology domains (aa 434 - 549 and 1261 - 1397), a Ras-binding region (aa 765 - 832) and a RhoGEF/DH domain (aa 1041 - 1232). Over aa 1 - 378, human TIAM1 shows 94% aa identity to mouse TIAM1.

Long Name

T-cell Lymphoma Invasion and Metastasis 1

Alternate Names

FLJ36302, human T-lymphoma invasion and metastasis inducing TIAM1 protein, 10TIAM-1, T-cell lymphoma invasion and metastasis 1, T-lymphoma invasion and metastasis-inducing protein 1

Entrez Gene IDs

7074 (Human); 21844 (Mouse); 304109 (Rat)

Gene Symbol

TIAM1

UniProt

Q13009

Additional TIAM1 Products

Product Documents for TIAM1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for TIAM1 Antibody

For research use only

Customer Reviews for TIAM1 Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
0%
3 Stars
67%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 的 3 reviews Showing All
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  • Human/Mouse TIAM1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018
  • Human/Mouse TIAM1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HCC44
    Species: Human
    Verified Customer | Posted 08/03/2018
    4% PFA fixation 1:100 dilution
    TIAM1 Antibody AF5038
  • Human/Mouse TIAM1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 human breast cancer cell line and SK-BR-3 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 07/23/2018
    Total cell lysates from MCF-7 and SK-BR-3 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human TIAM1 Antibody (AF5038). A specific band was detected for TIAM1 at approximately 180 kDa. This experiment was conducted under reducing conditions.
    TIAM1 Antibody AF5038

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