Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 67409
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Product Specifications
Immunogen
E. coli-derived recombinant human VEGF-B186
Accession # P49765
Accession # P49765
Specificity
Detects human VEGF-B186 in direct ELISAs and Western blots. In these formats, this antibody shows 100% cross‑reactivity with recombinant mouse (rm) VEGF-B186 but does not detect rh or rmVEGF-B167, rhVEGF206, rhVEGF167, rhVEGF-C, rhVEGF-D, rhCTGF, rhPlGF, or rhLDGF.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human/Mouse VEGF-B186 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse VEGF-B186 (Catalog # 767-VE)
Sample: Recombinant Mouse VEGF-B186 (Catalog # 767-VE)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: VEGF-B
Long Name
Vascular Endothelial Growth Factor B
Alternate Names
VEGFB
Gene Symbol
VEGFB
UniProt
Additional VEGF-B Products
Product Documents for Human/Mouse VEGF-B186 Antibody
Product Specific Notices for Human/Mouse VEGF-B186 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars