Human/Mouse WASF3/WAVE3 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5515R Datasheet / COA / SDS

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AF5515R-100UG

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All WASF3/WAVE3 Antibodies

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Human/Mouse WASF3/WAVE3 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human/mouse WASF3/WAVE3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human WASF3/WAVE3
Met1-Lys180
Accession # Q9UPY6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: WASF3/WAVE3

WASF3/WAVE3 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 3) is a 70 kDa member of the SCAR/WAVE family of proteins. It is expressed in select diverse cell types such as neurons, platelets, and breast epithelium. Upon activation, WASF3/WAVE3 is phosphorylated on Tyr151/248/337/486 and, in complex with HSPC300, initiates actin polymerization. WASF3/WAVE3 also regulates MMP expression. Human WASF3/WAVE3 is 502 amino acids (aa) in length. It contains one coiled-coil region (aa 57‑93), three sequential poly-Pro regions (aa 304‑410) and one VPH domain (aa 440‑457) that binds to actin and Arp2/3. There is one isoform that shows a 36 aa substitution for aa 181‑239. Over aa 1‑180, human WASF3 shares 98% aa identity with mouse WASF3.

Long Name

Wiskott-Aldrich Syndrome Protein Family Member 3

Entrez Gene IDs

10810 (Human); 245880 (Mouse)

Alternate Names

Brush-1; KIAA0900WASP family Verprolin-homologous protein 3; Protein WAVE-3; SCAR3; SCAR3WASP family protein member 3; Verprolin homology domain-containing protein 3; WAS protein family, member 3; WASF3; WAVE3; WAVE3Brush-1; wisk