Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY
View all Mre11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mre11
Glu423-Arg708
Accession # P49959

Specificity

Detects human Mre11 in Western blots.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for Human Mre11 Antibody

Detection of Human Mre11 antibody by Western Blot.

Detection of Human Mre11 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Mre11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2284) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for Mre11 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human Mre11 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from egg yolks

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mre11

The Mre11 protein exhibits both exo- and endonuclease activities and is a component of the Mre11/Rad50/Nbs1 (MRN) complex. The MRN complex binds chromatin in regions of DNA double-strand breaks (DSB) during the normal cell cycle and in response to genotoxic stress, and is involved in control of DSB repair during homologous recombination.

Long Name

Meiotic Recombination 11 Homolog A

Alternate Names

MRE11A

Entrez Gene IDs

4361 (Human)

Gene Symbol

MRE11

UniProt

P49959

Additional Mre11 Products

Product Documents for Human Mre11 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Mre11 Antibody

For research use only

Citations for Human Mre11 Antibody

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