Human NEDD9/CASL Alexa Fluor® 700-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: NEDD9/CASL

NEDD9 (also HEF1 and Cas-L) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta 1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length and contains one SH3 domain (aa 3‑65), a Tyr-rich SH2-binding region (aa 90‑350), one Ser-rich four alpha -helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710‑760). NEDD9 usually runs at 105 or 115 kDa in SDS‑PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155‑188, while two others show 20 and seven aa substitutions for aa 155‑834 and 1‑153, respectively. Over aa 153‑366, human and mouse NEDD9 share 85% aa identity.

Product Datasheets

Related Research Areas

• Actin Stress Fibers and Focal Adhesions During EMT
• Adhesion Molecules in Angiogenesis
• EMT Induction
• Focal Adhesions
• Integrins and Associated Molecules